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“SEE-DRUG ” is a FP7 - RESEARH POTENTIAL project that aims to the upgrade the Structural Biology capacities of UPAT and to the Establishment of a Center of Excellence for Structure-Based Drug Target chracterization efforts in South-Eastern EU region.
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“SEE-STRUCT” is structure-oriented module of SEE-DRUG project and aims to the structural elucidation of proteins, enzymes and other interesting drug-targets. The tools offered for these efforts are a state-of-the-art high field NMR instrument of 700 MHz equipped with a highly sensitive probe and crystallization robot.
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“SEE-PROT” & “SEE-PHARM” are the protein production and the preclinical evaluation of molecules, modules of SEE-DRUG project. The tools offered for these efforts are protein production and characterization facilities an upgraded confocal microscope, an intravital microscope and myographs.
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NMR
700MHz
High sensetivity probe
Studies in solution
Conformation/Dynamics
3D Structure determination, mobility, protein-protein interaction, protein-drug interactions
Confocal
In-cell imaging
Leica Microscope
Tandem Scanner
Additional laser
Sub-cellular ablation in live cells, protein-complexes, protein drug interactions in vivo
Robot
Crystal growth
Automation
Crystalization trials
Fast & efficient
Crystal growth optimization, screen of protein targes in paraller with NRM Spectroscopy
Microscopes
Myographs
intravital Microscope
In vivo
Ex vivo
Drug screening for anti-inflammatory compounds, evaluation of vasoactive compounds

Stuctural Biology
NMR & Xray
2H/13C/15N labeling
Selective labeling
Crystallization
Conformation dynamics, Modeling Structure determination, 3D solutions & crystal models, Bioinformatics
Molecular Biology
Biochemistry
Drug-targets
Biochemical pathways
Protein production
High-yield protein expression, labeling & isolation for structural studies, protein biochemistry
Imaging
In-cell studies
Functional imaging
Light Microscopy
FRET measurements
Studies of biomolecular functions through Advanced Light Microscopy, Sub-cellular ablations
Pharmacology
Inflammation
Vascular Biology
Cell-based assays
Signaling pathways
Monitoring cell processes (migration. proliferation, apoptosis), in vivo & ex vivo assays

Expertise Exchange
Twinnings
Knowledge transfer
Best practices
New applications
Exchange of researchers between UPAT and partnering organizations - EU Centers of excellence
Events
Workshops
Structural Biology
Biochemistry
Imaging
Workshops and scientific meetings will be organized at UPAT, Advanced training course in NMR
Dissemination
Conferences
Biomolecular NMR
Life Sciences
Analysis
Invited talks, Meeting participation & Poster presentations by SEE-DRUG members
Brokerage Events
Info-days
Meet the expers
Links with industry
Regional Authorities
Colloquia with invited speakers from academia or industry and private sector stakeholders

Expertise & Groups

The 13 UPAT Faculty members that participate in SEE-DRUG are affiliated with the Departments of Pharmacy, Medicine, Chemistry and Biology. They combine a publication record comprised of 650 publications, 25 published patents and >14000 citations.

Department of Pharmacy

  • Dr. Georgios A. Spyroulias, Assoc Prof. Expertise: NMR Spectroscopy, Protein production & characterisation, Structural Biology, Biomolecular Simulations.
  • Dr. Konstantinos Poulas, Assist. Prof. Expertise: Protein production & characterisation, X-ray Crystallography, Molecular modelling and Bioinformatics.
  • Dr. George P. Patrinos, Assist. Prof. Expertise: Human genomics, pharmacogenomics, Bioinformatics and genetic databases.


Recent publications

  1. Borg J, Georgitsi M, Patrinos GP, Philipsen S et al. Nature Genet 42, 801-5 (2010)
  2. Dalkas GA, Chassapis C, Spyroulias GA et al. Biochemistry 49, 10767-9 (2010)
  3. Georgitsi M, Poulas K, Tzimas G, Patrinos GP et al. Nucleic Acids Res 39(Database issue), D926-32 (2011)
  4. Abriata LA, Banci L, Bertini I, Gkazonis P, Spyroulias GA, et al. Nature Chem Biol 4, 599, (2008)
  5. Chen Z, Luo HY, Patrinos GP, et al. Mol Cell Biol 28, 4386-93, (2008)
  6. Banci L, Kandias NG, Robinson NJ, Spyroulias GA, et al. Proc Natl Acad Sci USA 103, 8320, (2006)


  • Prof. Andreas Papapetropoulos, , Prof. Expertise: Cellular Signalling, Protein-Protein Interactions, Cardiovascular Pharmacology, Inflammation, Pre-clinical drug screening & Development
  • Dr. StavrosTopouzis, Assist. Prof. Expertise: use of preclinical, cell-based (in vitro) and animal-based (in vivo) models, for the study of human fibroproliferative and inflammatory diseases.


Recent publications

  1. Papapetropoulos A, Pyriochou A, Altaany Z, Yang G, Marazioti A et al. Proc Natl Acad Sci USA 106:21972-7 (2009)
  2. Zhou, A. Pyriochou, A. Kotanidou, G. Dalkas, M.V. Eickels, G. Spyroulias, C. Roussos C and Papapetropoulos A. Am J Physiol Heart Circ Physiol. 295:H1763-71 (2008)
  3.  G. Haramis, Z. Zhou, A. Pyriochou, M. Koutsilieris, C. Roussos and A. Papapetropoulos. Br J Pharmacol. 155:804-13 (2008)
  4.  Z. Zhou, S. Gross, C. Roussos, S Meurer, W. Müller-Esterl and A. Papapetropoulos. J. Biol. Chem. 279:24935-24943, (2004)
  5. Dillon SR, Topouzis S et al. Nature Immunol, 5, 752-760 (2004)
  6. Papapetropoulos A, Zhou Z, Gerassimou C, Yetik G, Venema RC, Roussos C, et al. Mol Pharmacol. 68:1133-41 (2005)


Department of Medicine

  • Nicholas Moschonas, Prof. in Biology & Medical Molecular Genetics, expertise: protein production in bacteria and animal cells, gene expression and functional analysis of proteins, in vitro and cell-based assessment of protein-protein interactions
  • Zoi Lygerou, Assistant Professor in Biology, expertise: functional imaging, protein analysis in human cells and yeast, systems biology


Recent publications

  1. De Marco V, Lygerou Z, Taraviras S, Perrakis A. et al. Proc Natl Acad Sci USA, 106:19807-12 (2009)
  2. Lygeros J, Nurse P, Lygerou Z. et al. Proc Natl Acad Sci USA, 105:12295-12300 (2008)
  3. G. Xouri, Taraviras S, Bastiaens PIH, Lygerou Z. et al. EMBO J, 26:1303-1314 (2007)
  4. P. Deloukas, M. Kokkinaki, T. Sarafidou, N. K. Moschonas et al. Nature, 429, 375-381 (2004)
  5. H. Nishitani, Z. Lygerou, T. Nishimoto, P. Nurse. Nature 404:625-8 (2000)
  6. Z. Lygerou, P. Nurse P Science 290:2271-3 (2000)


  • Stavros Taraviras, Assistant Professor in Molecular and Cellular Pharmacology, Expertise: generation and analysis of genetically modified mice, isolation and culturing of embryonic haematopoietic and neural stem/progenitor cells, protein expression in different systems, biochemical characterisation of protein complexes, determination of gene and protein expression, live-cell imaging techniques.


Recent publications

  1. Karamitros D, Kioussis D, Taraviras S et al. J Immunol 184:2432-41 (2010)
  2. De Marco V, Lygerou Z, Taraviras S, Perrakis A. et al. Proc Natl Acad Sci USA 106:19807-12 (2009)
  3. Lygerou Z, Taraviras S, Papavassiliou AG, et al. Cancer Res. 67:10899-909 (2007)
  4. Roukos V, Taraviras S, Lygerou Z et al. J. Biol. Chem, 282:9346-9357 (2007)
  5. G. Xouri, Taraviras S, Bastiaens PIH, Lygerou Z. et al. EMBO J, 26:1303-1314 (2007)
  6. Bravou V, Taraviras S, et al. J Pathol. 208:91-9 (2006)


Department of Chemistry

  • John Matsoukas, Professor in Organic Chemistry. Expertise: Organic, Peptide synthesis and Characterization of Bioactive Molecules, Structure-Activity Relationship Studies.
  • Theodore Tselios, Assistant Professor in Organic Chemistry. Expertise: Rational Design, Synthesis & Characterization of Bioactive Molecules, Biomolecular and Docking Simulations.
  • Alexios Vlamis-Gardikas, Assistant Professor in Biochemistry, is a recently appointed faculty member. Expertise: Overexpression and purification of recombinant proteins, protein-protein interaction analysis, bacterial genetics, molecular biology, enzyme assays (especially those related to antioxidant enzymes).


Recent publications

  1. Katsara M, Tselios T, Matsoukas J, Apostolopoulos V et al. J Med Chem. 52:214-8 (2009).
  2. Deraos G, Tselios T, Apostolopoulos V, Matsoukas J. et al. J Med Chem. 51:7834-4 (2008).
  3. Spyranti Z, Matsoukas JM, Tselios TV et al. J Med Chem. 50:6039-47 (2007).
  4. Keramida MK, Tselios T, Matsoukas J. et al. J Med Chem. 49:105-10 (2006).
  5. Fernandes AP, Vlamis-Gardikas A. et al J Biol Chem. 280:24544-52 (2005).
  6. Fladvad M, Vlamis-Gardikas A, Sunnerhagen M et al. J Biol Chem. 280:24553-61 (2005).


Department of Biology

  • Kostas Flytzanis, Assoc. Prof. in Cell Biology expertise: gene regulation in early embryonic development of the sea urchin Paracentrotus lividus, study of the transcription factors and SAR studies on nuclear receptors (e.g. COUP-TF; Chicken Ovalbumin Upstream Promoter–Transcription Factors)
  • Irene Margiolaki, Lecturer in Structural Biology, Is a recently elected faculty member; expertise: in method development and application of Crystallography & Structural Biology. Extensive knowledge of powder and single crystal diffraction, structure determination, model building, refinement, and skills in Small Angle X-ray Scattering, optical/electron microscopy etc.


Recent publications

  1. Ahnfeldt T, Margiolaki I et al. Angew Chem Int Ed 48:5163-5166 (2009).
  2. Miller SR, Margiolaki I et al. J Am Chem Soc 130:15967-81 (2008).
  3. Margiolaki I, Wright JP, Wilmanns M, Fitch AN, Pinotsis N. J Am Chem Soc 129:11865-71 (2007).
  4. Sea Urchin Genome Sequencing Consortium (Flytzanis CN et al.) Science 314:941-952 (2006).
  5. Férey G, Mellot-Draznieks C, Serre C, Millange F, Dutour J, Surblé S, Margiolaki I Science 309:2040-2 (2005).
  6. Konstantopoulos AK, Flytzanis CN Mol Repro & Dev. 60:147-157 (2001).


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  • 01 Jan 2012 Starting data of SEE-DRUG project

  • 23 Feb 2012 Kick-off meeting

  • 23 Apr2012 External Advisory Board meeting

  • 29 Apr 2013 700MHz NMR installed