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“SEE-DRUG ” is a FP7 - RESEARH POTENTIAL project that aims to the upgrade the Structural Biology capacities of UPAT and to the Establishment of a Center of Excellence for Structure-Based Drug Target chracterization efforts in South-Eastern EU region.
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“SEE-STRUCT” is structure-oriented module of SEE-DRUG project and aims to the structural elucidation of proteins, enzymes and other interesting drug-targets. The tools offered for these efforts are a state-of-the-art high field NMR instrument of 700 MHz equipped with a highly sensitive probe and crystallization robot.
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“SEE-PROT” & “SEE-PHARM” are the protein production and the preclinical evaluation of molecules, modules of SEE-DRUG project. The tools offered for these efforts are protein production and characterization facilities an upgraded confocal microscope, an intravital microscope and myographs.
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NMR
700MHz
High sensetivity probe
Studies in solution
Conformation/Dynamics
3D Structure determination, mobility, protein-protein interaction, protein-drug interactions
Confocal
In-cell imaging
Leica Microscope
Tandem Scanner
Additional laser
Sub-cellular ablation in live cells, protein-complexes, protein drug interactions in vivo
Robot
Crystal growth
Automation
Crystalization trials
Fast & efficient
Crystal growth optimization, screen of protein targes in paraller with NRM Spectroscopy
Microscopes
Myographs
intravital Microscope
In vivo
Ex vivo
Drug screening for anti-inflammatory compounds, evaluation of vasoactive compounds

Stuctural Biology
NMR & Xray
2H/13C/15N labeling
Selective labeling
Crystallization
Conformation dynamics, Modeling Structure determination, 3D solutions & crystal models, Bioinformatics
Molecular Biology
Biochemistry
Drug-targets
Biochemical pathways
Protein production
High-yield protein expression, labeling & isolation for structural studies, protein biochemistry
Imaging
In-cell studies
Functional imaging
Light Microscopy
FRET measurements
Studies of biomolecular functions through Advanced Light Microscopy, Sub-cellular ablations
Pharmacology
Inflammation
Vascular Biology
Cell-based assays
Signaling pathways
Monitoring cell processes (migration. proliferation, apoptosis), in vivo & ex vivo assays

Expertise Exchange
Twinnings
Knowledge transfer
Best practices
New applications
Exchange of researchers between UPAT and partnering organizations - EU Centers of excellence
Events
Workshops
Structural Biology
Biochemistry
Imaging
Workshops and scientific meetings will be organized at UPAT, Advanced training course in NMR
Dissemination
Conferences
Biomolecular NMR
Life Sciences
Analysis
Invited talks, Meeting participation & Poster presentations by SEE-DRUG members
Brokerage Events
Info-days
Meet the expers
Links with industry
Regional Authorities
Colloquia with invited speakers from academia or industry and private sector stakeholders

Infrastructure

One of the main axes of the SEE-DRUG Action Plan proposal principal axis is the establishment of an S&T Infrastructure to face new challenges in chemical and structural biology, drug design and preclinical drug screening. Therefore, SEE-DRUG Action plan has as a major axe the Upgrade and Renewal of the S&T Equipment of the UPAT facilities on protein production, NMR instrumentation, crystallization and complementary techniques, such as imaging, of great importance in the study of protein-protein/small molecule interactions and pharmacology.

The following activities are foreseen:

Upgrade the UPAT NMR capacity in Structural Biology by acquisition of a state-of-the-art high-field NMR Spectrometer with enhanced sensitivity for efficient screening of peptides and proteins prior to NMR studies, crystallization efforts and X-ray structural studies.
A high-field NMR instrument, such as a 700 MHz NMR with a cryogenically cooled probe for enhanced sensitivity, in concert with uniformly or selectively enriched recombinant proteins (in 13C, 15N and 2H), will enable SEE-DRUG members, national and regional research groups to study large proteins with molecular weight above 10 kDa.


NMR Room at the new Pharmacy Builging

Acquisition of a Crystallization Robot, which will be coupled with the NMR facility. Initial screening of protein-targets with NMR (to confirm, folding, oligomerization state, solubility etc.) will be followed by crystallization screening conditions in order to produce protein-substrate/drug complexes. The coupling of the two instruments will be time and cost-effective since it will reduce the false attempts to crystallize structureless or self-aggregating proteins.

Upgrade of the UPAT Advance Light Microscopy Facility. Upgrading of the existing Confocal Microscopy Facility, will comprise, according to our knowledge, a unique facility in Greece for its capacities, properties and applications. This equipment will be operated by an expert in confocal and functional microscopy, in collaboration with experts in molecular biology, in NMR and in in vivo assays.

Upgrade the UPAT molecular pharmacology laboratory bioactivity testing capacity with an Intravital Microscope and a set of Myographs.


Light Imaging Facility – Confocal microscope installed
at the lab of UPAT’s Medical School

Light Imaging Facility – Confocal microscope
installed at the lab of UPAT’s Medical School


Myographs (Dept of Pharmacy, UPAT)

Crystallization Robot installed at the UPAT’s Pharmacy Department











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  • 01 Jan 2012 Starting data of SEE-DRUG project

  • 23 Feb 2012 Kick-off meeting

  • 23 Apr2012 External Advisory Board meeting

  • 29 Apr 2013 700MHz NMR installed