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“SEE-DRUG ” is a FP7 - RESEARH POTENTIAL project that aims to the upgrade the Structural Biology capacities of UPAT and to the Establishment of a Center of Excellence for Structure-Based Drug Target chracterization efforts in South-Eastern EU region.
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“SEE-STRUCT” is structure-oriented module of SEE-DRUG project and aims to the structural elucidation of proteins, enzymes and other interesting drug-targets. The tools offered for these efforts are a state-of-the-art high field NMR instrument of 700 MHz equipped with a highly sensitive probe and crystallization robot.
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“SEE-PROT” & “SEE-PHARM” are the protein production and the preclinical evaluation of molecules, modules of SEE-DRUG project. The tools offered for these efforts are protein production and characterization facilities an upgraded confocal microscope, an intravital microscope and myographs.
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NMR
700MHz
High sensetivity probe
Studies in solution
Conformation/Dynamics
3D Structure determination, mobility, protein-protein interaction, protein-drug interactions
Confocal
In-cell imaging
Leica Microscope
Tandem Scanner
Additional laser
Sub-cellular ablation in live cells, protein-complexes, protein drug interactions in vivo
Robot
Crystal growth
Automation
Crystalization trials
Fast & efficient
Crystal growth optimization, screen of protein targes in paraller with NRM Spectroscopy
Microscopes
Myographs
intravital Microscope
In vivo
Ex vivo
Drug screening for anti-inflammatory compounds, evaluation of vasoactive compounds

Stuctural Biology
NMR & Xray
2H/13C/15N labeling
Selective labeling
Crystallization
Conformation dynamics, Modeling Structure determination, 3D solutions & crystal models, Bioinformatics
Molecular Biology
Biochemistry
Drug-targets
Biochemical pathways
Protein production
High-yield protein expression, labeling & isolation for structural studies, protein biochemistry
Imaging
In-cell studies
Functional imaging
Light Microscopy
FRET measurements
Studies of biomolecular functions through Advanced Light Microscopy, Sub-cellular ablations
Pharmacology
Inflammation
Vascular Biology
Cell-based assays
Signaling pathways
Monitoring cell processes (migration. proliferation, apoptosis), in vivo & ex vivo assays

Expertise Exchange
Twinnings
Knowledge transfer
Best practices
New applications
Exchange of researchers between UPAT and partnering organizations - EU Centers of excellence
Events
Workshops
Structural Biology
Biochemistry
Imaging
Workshops and scientific meetings will be organized at UPAT, Advanced training course in NMR
Dissemination
Conferences
Biomolecular NMR
Life Sciences
Analysis
Invited talks, Meeting participation & Poster presentations by SEE-DRUG members
Brokerage Events
Info-days
Meet the expers
Links with industry
Regional Authorities
Colloquia with invited speakers from academia or industry and private sector stakeholders

Journals

Activities accomplished:

(I) Oral presentations:                                                              22 (+37) =        Total 59

(II) Participation in Conferences and Large Scale RIs meetings:       53 (+26) =        Total 79

(III) Scientific publications, in peer-reviewed journals:                   29 (+10) =        Total 39

(IV) Articles in newspapers, journals & newsletters                       5 (+20) =          Total 25

Publications in peer-reviewing scientific journals, by SEE-DRUG groups:

  1. NMR-based insights into the conformational and interaction properties of Arkadia RING-H2 E3 Ub ligase. Chasapis CT, Kandias NG, Episkopou V, Bentrop D, Spyroulias GA. Proteins. 2012 May;80(5):1484-9.
  2. Hydrogen sulfide and nitric oxide are mutually dependent in the regulation of angiogenesis and endothelium-dependent vasorelaxation. Coletta C, Papapetropoulos A, Erdelyi K, Olah G, Módis K, Panopoulos P, Asimakopoulou A, Gerö D, Sharina I, Martin E, Szabo C. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):9161-6.
  3. Structural studies of human insulin cocrystallized with phenol or resorcinol via powder diffraction. Karavassili F, Giannopoulou AE, Kotsiliti E, Knight L, Norrman M, Schluckebier G, Drube L, Fitch AN, Wright JP, Margiolaki I. Acta Crystallogr D Biol Crystallogr. 2012 Dec;68(Pt 12):1632-41
  4. (1)H, (15)N, (13)C assignment and secondary structure determination of two domains of La protein from D. discoideum. Apostolidi M, Vourtsis DJ, Chasapis CT, Stathopoulos C, Bentrop D, Spyroulias GA. Biomol NMR Assign. 2012 Dec 14.
  5. cGMP-dependent protein kinase contributes to hydrogen sulfide-stimulated vasorelaxation. M. Bucci, A. Papapetropoulos, V. Vellecco, Z. Zhou, A. Zaid, P. Giannogonas, A. Cantalupo, S. Dhayade, K.P. Karalis, R. Wang, R. Feil, G. Cirino. PlosOne 2012;7(12):e53319.
  6. Selectivity of commonly used pharmacological inhibitors for cystathionine β synthase (CBS) and cystathionine γ lyase (CSE). Asimakopoulou A, Panopoulos P, Chasapis CT, Coletta C, Zhou Z, Cirino G, Giannis A, Szabo C, Spyroulias GA, Papapetropoulos A. Br J Pharmacol 2013;169(4):922-32.
  7. Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A. K. Módis, P. Panopoulos, C. Coletta, A. Papapetropoulos, C. Szabo. Biochem. Pharm. 2013, 86:1311-9,
  8. High-resolution powder X-ray data reveal the T(6) hexameric form of bovine insulin. Margiolaki I, Giannopoulou AE, Wright JP, Knight L, Norrman M, Schluckebier G, Fitch AN, Von Dreele RB. Acta Crystallogr D Biol Crystallogr 2013;69(Pt 6):978-90. doi: 10.1107/S0907444913003867. Epub 2013 May 11.
  9. Genomic variation in the MAP3K5 gene is associated with β-thalassemia disease severity and hydroxyurea treatment efficacy. Tafrali C, Paizi A, Borg J, Radmilovic M, Bartsakoulia M, Giannopoulou E, Giannakopoulou O, Stojiljkovic-Petrovic M, Zukic B, Poulas K, Stavrou EF, Lambropoulou P, Kourakli A, Felice AE, Papachatzopoulou A, Philipsen S, Pavlovic S, Georgitsi M, Patrinos GP. Pharmacogenomic 2013;14(5):469-83.
  10. Insights into soluble guanylyl cyclase activation derived from improved heme-mimetics. von Wantoch Rekowski M, Kumar V, Zhou Z, Moschner J, Marazioti A, Bantzi M, Spyroulias GA, van den Akker F, Giannis A, Papapetropoulos A. J. Med. Chem. 2013, 56, 8948-52,
  11. Hydrogen sulfide accounts for the peripheral vascular effects of S-zofenopril independently of ACE inhibition. M. Bucci, V. Vellecco,  A.Cantalupo, V. Brancaleone, Z. Zhou, S. Evangelista,  V. Calderone,  A. Papapetropoulos, G. Cirino. Cardiovasc Res. 2014, 102:138-47,
  12. NMR conformational properties of an Anthrax Lethal Factor domain studied by multiple amino acid-selective labeling. Vourtsis DJ, Chasapis CT, Pairas G, Bentrop D, Spyroulias GA. Biochem Biophys Res Commun. 2014, 450(1):335-40.
  13. Regulation of Mitochondrial Bioenergetic Function by Hydrogen Sulfide. Part II. Pathophysiological and Therapeutic Aspects. Módis K, Bos EM, Calzia E, van Goor H, Coletta C, Papapetropoulos A, Hellmich MR, Radermacher P, Bouillaud F, Szabo C. Br J pharmacol. 2014 Apr;171(8):2123-46.
  14. NMR study of non-structural proteins-part II: 1H, 13C, 15N backbone and side-chain resonance assignment of macro domain from Venezuelan equine encephalitis virus (VEEV). Makrynitsa GI, Ntonti D, Marousis KD, Tsika AC, Lichière J, Papageorgiou N, Coutard B, Bentrop D, Spyroulias GA. Biomol NMR Assign. 2014 Oct 8. [Epub ahead of print]
  15. Backbone and side chain NMR assignment, along with the secondary structure prediction of RRM2 domain of La protein from a lower eukaryote exhibiting identical structural organization with its human homolog. Argyriou AI, Chasapis CT, Apostolidi M, Konstantinidou P, Stathopoulos C, Bentrop D, Spyroulias GA. Biomol NMR Assign. 2015 Apr;9(1):219-22.
  16. NMR study of non-structural proteins--part I: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Mayaro virus (MAYV). Melekis E, Tsika AC, Lichière J, Chasapis CT, Margiolaki I, Papageorgiou N, Coutard B, Bentrop D, Spyroulias GA. Biomol NMR Assign. 2015 Apr;9(1):191-5.
  17. 1H, 13C and 15N backbone and side-chain resonance assignment of the LAM-RRM1 N-terminal module of La protein from Dictyostelium discoideum. Chasapis CT, Argyriou AI, Apostolidi M, Konstantinidou P, Bentrop D, Stathopoulos C, Spyroulias GA. Biomol NMR Assign. 2015 Feb 18. [Epub ahead of print]
  18. Evaluation of the distribution of Paclitaxel by immunohistochemistry and nuclear magnetic resonance spectroscopy after the application of a drug-eluting balloon in the porcine ureter. Liourdi D, Kallidonis P, Kyriazis I, Tsamandas A, Karnabatidis D, Kitrou P, Spyroulias GA, Kostopoulou ON, Marousis K, Kalpaxis DL, Goumenos DS, Liatsikos E. J Endourol. 2015 May;29(5):580-9.
  19. A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D. Eggink LL, Spyroulias GA, Jones NG, Hanson CV, Hoober JK. PLoS One. 2015 Jun 25;10(6):e0130532. doi: 10.1371/journal.pone.0130532. eCollection 2015.
  20. NMR & Biochemical assays reveal unexpected RNA binding properties of the La motif of D. discoideum Lupus Antigen protein. A. Argyriou, D. Vourtsis, M. Apostolidi, C.T. Chasapis, D. Drainas, D. Bentrop, C. Stathopoulos, G.A. Spyroulias. (2015) to be submitted 
  21. NMR insights on the conformational plasticity and ligase activity of intrinsically dynamical forms of ARKADIA E3 Ubiquitin Ligase. M. Birkou, A. Loutsidou, C.T. Chasapis, D. Bentrop, M. Leli, T. Herrmann, V. Episkopou, G.A. Spyroulias. (2015) to be submitted
  22. Role of cGMP in hydrogen sulfide signaling. SI Bibli, G. Yang, Z. Zhou, R.Wang, S. Topouzis, A. PapapetropoulosNO Journal 2015, 30:46:7-13,
  23. Extending the translational potential of targeting nitric oxide/cGMP-regulated pathways in the cardiovascular system. A. Papapetropoulos, A. J. Hobbs, S. Topouzis. Br. J. Pharmacol 2015, 172:1397-414,
  24. Direct proof of the in vivo pathogenic role of the AChR autoantibodies from myasthenia gravis patients. Kordas G, Lagoumintzis G, Sideris S, Poulas K, Tzartos SJ. PLoS One. 2014 Sep 26;9(9):e108327.
  25. Scale up and safety parameters of antigen specific immunoadsorption of human anti-acetylcholine receptor antibodies. Lagoumintzis G, Zisimopoulou P, Trakas N, Grapsa E, Poulas K, Tzartos SJ. J Neuroimmunol. 2014 Feb 15;267(1-2):1-6.
  26. Non-contact current transfer induces the formation and improves the X-ray diffraction quality of protein crystals. Boltsis I, Lagoumintzis G, Chatzileontiadou DSM, Giastas P, Tzartos SJ, D. Leonidas D, Poulas K. Journal of Crystal Growth and Design, 2014, 14(9): 4347-4354.
  27. ATP-Sensitive Potassium Channel Activation Induces Angiogenesis In Vitro and In Vivo. Umaru B, Pyriochou A, Kotsikoris V, Papapetropoulos A, Topouzis S. J Pharmacol Exp Ther. 2015, Jul;354(1):79-87.
  28. Guanylyl cyclase activation reverses resistive breathing-induced lung injury and inflammation. Glynos C, Toumpanakis D, Loverdos K, Karavana V, Zhou Z, Magkou C, Dettoraki M, Perlikos F, Pavlidou A, Kotsikoris V, Topouzis S, Theocharis SE, Brouckaert P, Giannis A, Papapetropoulos A, Vassilakopoulos T. Am J Respir Cell Mol Biol. 2015 Jun;52(6):762-71.
  29. Mechanoresponses of human primary osteoblasts grown on carbon nanotubes. Kroustalli A, Kotsikoris V, Karamitri A, Topouzis S, Deligianni D. J Biomed Mater Res A. 2015 Mar;103(3):1038-44.
  30. Mast cells mediate malignant pleural effusion formation. Giannou AD, Marazioti A, Spella M, Kanellakis NI, Apostolopoulou H, Psallidas I, Prijovich ZM, Vreka M, Zazara DE, Lilis I, Papaleonidopoulos V, Kairi CA, Patmanidi AL, Giopanou I, Spiropoulou N, Harokopos V, Aidinis V, Spyratos D, Teliousi S, Papadaki H, Taraviras S, Snyder LA, Eickelberg O, Kardamakis D, Iwakura Y, Feyerabend TB, Rodewald HR, Kalomenidis I, Blackwell TS, Agalioti T, Stathopoulos GT. J Clin Invest. 2015 Jun 1;125(6):2317-34.
  31. Geminin deletion increases the number of fetal hematopoietic stem cells by affecting the expression of key transcription factors. Karamitros D, Patmanidi AL, Kotantaki P, Potocnik AJ, Bähr-Ivacevic T, Benes V, Lygerou Z, Kioussis D, Taraviras S. Development. 2015 Jan 1;142(1):70-81.
  32. “Human Insulin Polymorphism upon Ligand Binding and pH variation: The Case of 4-ethylresorcinol.” S. Fili, A.Valmas, M. Norrman, G. Schluckebier, D. Beckers, T. Degen, J. Wright, A. Fitch, F. Gozzo, A. E. Giannopoulou, F. Karavassili, I. Margiolaki. (Submitted in IUCrJ, 2015).
  33. “Novel crystalline phase and first-order phase transitions of human insulin complexed with two distinct phenol derivatives”. Valmas, A., Magiouf, K., Fili, S., Norrman, M., Schluckebier, G., Beckers, D., Degen, T., Wright, J.P., Fitch, A. N., Gozzo, F., Giannopoulou, A.E., Karavassili, F. Margiolaki I. Acta Cryst. 2015, D71, 819-828.
  34. “The power of Powders”, Article on ESRFnews magazine, (vol. 69, page 18). Karavassili, F., Valmas, A., Margiolaki, I., (2015).
  35. Utilizing the virus-induced blocking of apoptosis in an easy baculovirus titration method. A. Niarchos, G. Lagoumintzis and K. Poulas. In press, Scientific Reports, Nature Publishing Group, 2015.

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  • 01 Jan 2012 Starting data of SEE-DRUG project

  • 23 Feb 2012 Kick-off meeting

  • 23 Apr2012 External Advisory Board meeting

  • 29 Apr 2013 700MHz NMR installed