image image image

“SEE-DRUG ” is a FP7 - RESEARH POTENTIAL project that aims to the upgrade the Structural Biology capacities of UPAT and to the Establishment of a Center of Excellence for Structure-Based Drug Target chracterization efforts in South-Eastern EU region.
Read the Full Story

“SEE-STRUCT” is structure-oriented module of SEE-DRUG project and aims to the structural elucidation of proteins, enzymes and other interesting drug-targets. The tools offered for these efforts are a state-of-the-art high field NMR instrument of 700 MHz equipped with a highly sensitive probe and crystallization robot.
Read the Full Story

“SEE-PROT” & “SEE-PHARM” are the protein production and the preclinical evaluation of molecules, modules of SEE-DRUG project. The tools offered for these efforts are protein production and characterization facilities an upgraded confocal microscope, an intravital microscope and myographs.
Read the Full Story

High sensetivity probe
Studies in solution
3D Structure determination, mobility, protein-protein interaction, protein-drug interactions
In-cell imaging
Leica Microscope
Tandem Scanner
Additional laser
Sub-cellular ablation in live cells, protein-complexes, protein drug interactions in vivo
Crystal growth
Crystalization trials
Fast & efficient
Crystal growth optimization, screen of protein targes in paraller with NRM Spectroscopy
intravital Microscope
In vivo
Ex vivo
Drug screening for anti-inflammatory compounds, evaluation of vasoactive compounds

Stuctural Biology
NMR & Xray
2H/13C/15N labeling
Selective labeling
Conformation dynamics, Modeling Structure determination, 3D solutions & crystal models, Bioinformatics
Molecular Biology
Biochemical pathways
Protein production
High-yield protein expression, labeling & isolation for structural studies, protein biochemistry
In-cell studies
Functional imaging
Light Microscopy
FRET measurements
Studies of biomolecular functions through Advanced Light Microscopy, Sub-cellular ablations
Vascular Biology
Cell-based assays
Signaling pathways
Monitoring cell processes (migration. proliferation, apoptosis), in vivo & ex vivo assays

Expertise Exchange
Knowledge transfer
Best practices
New applications
Exchange of researchers between UPAT and partnering organizations - EU Centers of excellence
Structural Biology
Workshops and scientific meetings will be organized at UPAT, Advanced training course in NMR
Biomolecular NMR
Life Sciences
Invited talks, Meeting participation & Poster presentations by SEE-DRUG members
Brokerage Events
Meet the expers
Links with industry
Regional Authorities
Colloquia with invited speakers from academia or industry and private sector stakeholders

Robot Crystal growth

A crystallization robot, unique in UPAT, is expected to provide new possibilities for crystallization trials (SEE-STRUCT module) and enhance efforts in Structure determination of proteins in crystals through X-ray diffraction.

Protein production and purification requires usually heavy efforts and loss of protein quantities. Therefore, pure protein is expensive and often hundreds of combinations of variables must be tried before a novel protein can be crystallized from solution. Manual methods for crystallization are slow and error-prone.

UPAT’s Crystallization Robot provides the possibility for quick and accurate dispense of vapour diffusion drops from pre-dispensed reservoirs into sitting drop wells. Protein can be added at the same time using automated dispensing techniques. A suite of software includes packages for screening and optimization and provides easy-to-use interfaces for setting up screening experiments. Algorithms calculate and control pH for any number of buffer species. It can deliver more than 350 microbatch wells per hour, automatically covered with oil with no further requirement for sealing. This equipment will enhance the structural determination efforts of the SEE-DRUG initiatives, being integrated between the SEE-PROT/STRUCT/PHARM groups. With this tool a large number of proteins (either produced by the members of the SEE-DRUG project or by non-UPAT collaborators) will be tested for crystallization. Additionally, the dynamic information obtained by the NMR methodology will be used for the successful crystallization of proteins.

Successful crystals will be used in diffractometers in Athens or in European Synchrotrons facilities for high-resolution structure determination.

Upcoming Events

Hot off the Press

porn /div> The NMR Structure of ARKADIA RING Domain (Proteins 2012, 50, 1484-89)

H2S and NO in angiogenesis & vasodilatation (PNAS 2012 in the press)

Site Statistics

mod_vvisit_counterThis week92
mod_vvisit_counterLast week204
mod_vvisit_counterThis month1835
mod_vvisit_counterLast month822
mod_vvisit_counterAll days262126

We have: 5 guests online
Your IP:
Today: Apr 23, 2024
University of Patras General Info, Services, Research Committee, etc.

FP7 Research & Innovation – Participants Portal – Calls for applications

International Meetings & Conferences

Journal Club

  • 01 Jan 2012 Starting data of SEE-DRUG project

  • 23 Feb 2012 Kick-off meeting

  • 23 Apr2012 External Advisory Board meeting

  • 29 Apr 2013 700MHz NMR installed