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“SEE-DRUG ” is a FP7 - RESEARH POTENTIAL project that aims to the upgrade the Structural Biology capacities of UPAT and to the Establishment of a Center of Excellence for Structure-Based Drug Target chracterization efforts in South-Eastern EU region.
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“SEE-STRUCT” is structure-oriented module of SEE-DRUG project and aims to the structural elucidation of proteins, enzymes and other interesting drug-targets. The tools offered for these efforts are a state-of-the-art high field NMR instrument of 700 MHz equipped with a highly sensitive probe and crystallization robot.
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“SEE-PROT” & “SEE-PHARM” are the protein production and the preclinical evaluation of molecules, modules of SEE-DRUG project. The tools offered for these efforts are protein production and characterization facilities an upgraded confocal microscope, an intravital microscope and myographs.
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NMR
700MHz
High sensetivity probe
Studies in solution
Conformation/Dynamics
3D Structure determination, mobility, protein-protein interaction, protein-drug interactions
Confocal
In-cell imaging
Leica Microscope
Tandem Scanner
Additional laser
Sub-cellular ablation in live cells, protein-complexes, protein drug interactions in vivo
Robot
Crystal growth
Automation
Crystalization trials
Fast & efficient
Crystal growth optimization, screen of protein targes in paraller with NRM Spectroscopy
Microscopes
Myographs
intravital Microscope
In vivo
Ex vivo
Drug screening for anti-inflammatory compounds, evaluation of vasoactive compounds

Stuctural Biology
NMR & Xray
2H/13C/15N labeling
Selective labeling
Crystallization
Conformation dynamics, Modeling Structure determination, 3D solutions & crystal models, Bioinformatics
Molecular Biology
Biochemistry
Drug-targets
Biochemical pathways
Protein production
High-yield protein expression, labeling & isolation for structural studies, protein biochemistry
Imaging
In-cell studies
Functional imaging
Light Microscopy
FRET measurements
Studies of biomolecular functions through Advanced Light Microscopy, Sub-cellular ablations
Pharmacology
Inflammation
Vascular Biology
Cell-based assays
Signaling pathways
Monitoring cell processes (migration. proliferation, apoptosis), in vivo & ex vivo assays

Expertise Exchange
Twinnings
Knowledge transfer
Best practices
New applications
Exchange of researchers between UPAT and partnering organizations - EU Centers of excellence
Events
Workshops
Structural Biology
Biochemistry
Imaging
Workshops and scientific meetings will be organized at UPAT, Advanced training course in NMR
Dissemination
Conferences
Biomolecular NMR
Life Sciences
Analysis
Invited talks, Meeting participation & Poster presentations by SEE-DRUG members
Brokerage Events
Info-days
Meet the expers
Links with industry
Regional Authorities
Colloquia with invited speakers from academia or industry and private sector stakeholders

Microscopes Myographs

Intravital Microscope:

This setup allows us to visualize, in anesthetized animals in vivo, the interaction of leukocytes with the blood vessel wall and the crossing of the blood-vessel interface (emigration). This complex interaction is crucial in many diseases with an inflammatory component, for example during the acute phase in asthma and the chronic progression observed in atherosclerosis. Typically, the microcirculation of a specific bed is exposed under microscope and the interactions of leukocytes with the endothelial cells lining the blood vessel can be observed, recorded and quantitated. Parameters that are quantifiable are rolling speed or rolling influx of circulating leukocytes, leukocyte number per square mm and emigrated leukocytes, together elucidating the specific step which a chemical may target and thus influence critical processes of inflammation.

Myographs:

Altered vascular tone is frequently a crucial denominator or a severely complicating factor of the vascular dysfunction that characterizes many major diseases, such as hypertension, atherosclerosis, sepsis, Alzheimer’s, myocardial infarction and diabetic arteriopathy. The tone of the vessel is essentially determined in tandem by the endothelium and the smooth muscle cells. A direct measurement of how various hormones and chemicals influence vessel tone is therefore vital for understanding how these will impact vascular contractility (tone) and influence the progress of a particular disease. Myographs, for a few decades now, have been successfully used to: a) test the contractile activity of hormones, neurotransmitters and chemicals on the arterial wall, with added advantage that newer generation myographs allow the use of both larger calibre as well as smaller, resistance vessels, b) elucidate the molecular mechanism of action of many compounds, through chemical manipulation by use of agonists, antagonists and signalling pathway modulators, and c) functionally screen compounds, if their target is expressed in the vessel wall. It will therefore be an important addition to our arsenal in screening for compounds with effects on the cardiovascular system. Furthermore, it will allow comparison of vascular reactivity in vessels from wild-type and genetically manipulated (knock-out or transgenic) mice, which carry altered gene expression in the vasculature. Finally, this versatile tool can be used to measure reactivity in tracheal preparations, thus expanding its utility in investigations in pulmonary disease models.

Upcoming Events

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  • 01 Jan 2012 Starting data of SEE-DRUG project

  • 23 Feb 2012 Kick-off meeting

  • 23 Apr2012 External Advisory Board meeting

  • 29 Apr 2013 700MHz NMR installed