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“SEE-DRUG ” is a FP7 - RESEARH POTENTIAL project that aims to the upgrade the Structural Biology capacities of UPAT and to the Establishment of a Center of Excellence for Structure-Based Drug Target chracterization efforts in South-Eastern EU region.
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“SEE-STRUCT” is structure-oriented module of SEE-DRUG project and aims to the structural elucidation of proteins, enzymes and other interesting drug-targets. The tools offered for these efforts are a state-of-the-art high field NMR instrument of 700 MHz equipped with a highly sensitive probe and crystallization robot.
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“SEE-PROT” & “SEE-PHARM” are the protein production and the preclinical evaluation of molecules, modules of SEE-DRUG project. The tools offered for these efforts are protein production and characterization facilities an upgraded confocal microscope, an intravital microscope and myographs.
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NMR
700MHz
High sensetivity probe
Studies in solution
Conformation/Dynamics
3D Structure determination, mobility, protein-protein interaction, protein-drug interactions
Confocal
In-cell imaging
Leica Microscope
Tandem Scanner
Additional laser
Sub-cellular ablation in live cells, protein-complexes, protein drug interactions in vivo
Robot
Crystal growth
Automation
Crystalization trials
Fast & efficient
Crystal growth optimization, screen of protein targes in paraller with NRM Spectroscopy
Microscopes
Myographs
intravital Microscope
In vivo
Ex vivo
Drug screening for anti-inflammatory compounds, evaluation of vasoactive compounds

Stuctural Biology
NMR & Xray
2H/13C/15N labeling
Selective labeling
Crystallization
Conformation dynamics, Modeling Structure determination, 3D solutions & crystal models, Bioinformatics
Molecular Biology
Biochemistry
Drug-targets
Biochemical pathways
Protein production
High-yield protein expression, labeling & isolation for structural studies, protein biochemistry
Imaging
In-cell studies
Functional imaging
Light Microscopy
FRET measurements
Studies of biomolecular functions through Advanced Light Microscopy, Sub-cellular ablations
Pharmacology
Inflammation
Vascular Biology
Cell-based assays
Signaling pathways
Monitoring cell processes (migration. proliferation, apoptosis), in vivo & ex vivo assays

Expertise Exchange
Twinnings
Knowledge transfer
Best practices
New applications
Exchange of researchers between UPAT and partnering organizations - EU Centers of excellence
Events
Workshops
Structural Biology
Biochemistry
Imaging
Workshops and scientific meetings will be organized at UPAT, Advanced training course in NMR
Dissemination
Conferences
Biomolecular NMR
Life Sciences
Analysis
Invited talks, Meeting participation & Poster presentations by SEE-DRUG members
Brokerage Events
Info-days
Meet the expers
Links with industry
Regional Authorities
Colloquia with invited speakers from academia or industry and private sector stakeholders

Workshop: Peptide Synthesis and Protein production/labeling

Organizing Committee:
Kostas Poulas
Stavros Topouzis
Kostas Flytzanis
John Matsoukas
Georgios Spyroulias

Conference & Cultural Center
University of Patras
Amphitheater I-4

WEDNSESDAY 9th MAY2012
15:30 – 17:15
1st Session: Bioactive Peptides & Proteins

Chairpersons

J. Matsoukas (Patras, Hellas) & I. Margiolaki (Patras, Hellas)

15:3016:05The importance of a “Chemical Reverse Approach” to modulate peptide secondary structures inducing specific and high affinity biological recognition
Prof. Anna-Maria Papini
Department of Chemistry Ugo Schiff, University of Florence, Italy
16:05 – 16:35

Regulation of MDM2 Function by p110b/LSD1
Prof. Nikolaos Ar. Papanikolaou
Department of Medicine, Aristotle University of Thesaloniki, Hellas

16:3516:55

Microwave-assisted approach in solid-phase peptide synthesis: case study of PTHrP(1-34)NH2
Dr. Fabio Rizzolo

Universite de Cergy-Pontoise, France

16:55 – 17:10Coffee Break
17:10 – 18:502nd Session: Peptides and Protein Production for Structural Studies
Chairpersons

 F. Rizzolo (Florence, Italy) & T. Tselios (Patras, Hellas)


17:10 – 17:45

An unorthodox molecular approach to the targeting of proteins in vitro and in vivo using synthetic polypeptides conjugated to small organic molecules.
Prof. Lars Baltzer
Department of Biochemistry, University of Upsala, Sweden

17:45 – 18:10

Macromolecular Powder Diffraction: Current Status & Future Prospect
Dr. Irene Margiolaki
Department of Biology, University of Patras, Hellas

18:10 – 18:30

Protein production & labeling strategies for Biomolecular NMR studies
Dr. Christos Chasapis
Department of Pharmacy, University of Patras, Hellas

18:30 – 18:50

Expression of Cys-loop receptors in prokaryotic & eukaryotic systems
Dr. George Lagoumintzis
Department of Pharmacy, University of Patras, Hellas



20:30 – 22:30Social Event  -  Cultural & Conference Center of the University of Patras


THURSDAY 10th MAY2012
09:45 – 11:503rd Session: Protein Production in Prokaryotic & Eukaryotic systems
Chairpersons

 A. Papapetropoulos (Patras, Hellas) & S. Topouzis (Patras, Hellas)

09:45 – 10:20

Smelling Vibrations
Dr. Efthimios Skoulakis

B.S.R.C. "Alexander Fleming", Varkiza,  Hellas

10:20 – 10:55

Multiple functions of polysaccharide deacetylases from Bacillus anthracis
Prof. Vasilios Bouriotis

Department of Biology, University of Crete, Hellas

10:55 – 11:30

Insect cell-based expression technologies as tools for structural and functional analysis of eukaryotic proteins
Dr. Luc L.H. Swevers
NCSR Demokritos, Athens, Hellas

11:30 – 11:50

Antisense Masking Reveals Contributions of mRNA-rRNA Base Pairing to Translation of GTX and FGF2 mRNAs
Dr. P. Panopoulos
Department of Pharmacy, University of Patras, Hellas

11:50 – 12:10Coffee Break
12:10 – 13:504th Session:Protein Production & Structural Studies of Biomolecules
ChairpersonsK. Poulas (Patras, Hellas) & G.A. Spyroulias (Patras, Hellas)
12:10 – 12:45

General principles of heterologous protein production systems – A case study
Prof. Costas Vorgias
Department of Biology, Kapodistrian University of Athens, Hellas

12:45 – 13:20

Mechanism of activity regulation in calcium/calmodulin regulated protein kinases
Dr. Koen Temmerman
EMBL- Centre for Cellular and Molecular Imaging, Hamburg, Germany

13:20 – 13:50

Structural biology sheds light on biocatalysts function
Dr. Evangelia Chryssina
National Hellenic Research Foundation (NHRF), Athens, Hellas

13:50 – 15:30Light lunch & Coffee
15:30 – 16:50FP7 Info Session
ChairpersonG.A. Spyroulias  (Patras, Hellas)

New FP7 calls in HEALTH, IDEAS (for health Sciences), Marie Curie  & Enterprise Europe Network-Hellas  -  Discussion
Mrs Christina Pascual & Georgia Tzenou
National Documentation Center & National Contact Point, NHRF, Athens, Hellas

16:30 – 18:055th Session: Structure-Activity Correlation Studies of Biomolecules
ChairpersonsK. Poulas     (Patras, Hellas), T. Tselios    (Patras, Hellas)
16:30 – 17:05

Protein Modules Involved in Chromatin Remodeling: Structures and Interactions with Chromatin Components
Prof. Anastasia Politou
School of Medicine, University of Ioannina, Hellas

17:05 – 17:20

Lessons from the small RNAs role in plant biochemistry for human diseases’ cure
Dr. Christos K. Kotakis
Department of Biology, University of Crete, Hellas

17:20 – 17:35

Crucial role of granulocytic myeloid-derived suppressor cells in the regulation of central nervous system autoimmune disease
Dr. Panagiotis Verginis
Medical School, University of Crete, Hellas

17:35 – 17:50

The Nrf2 antioxidant system in the thyroid gland
Dr. Gerasimos Sykiotis
Medical School, University of Patras, Hellas

17:50 – 18:05

Future Networking Activities of the SEE-DRUG project – The 2nd SEE-DRUG Workshop
K. Poulas, & I. Margiolaki


You can download the program of the meeting in PDF format here

Z. Lygerou  (Dept of Medicine)

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  • 01 Jan 2012 Starting data of SEE-DRUG project

  • 23 Feb 2012 Kick-off meeting

  • 23 Apr2012 External Advisory Board meeting

  • 29 Apr 2013 700MHz NMR installed