image image image

“SEE-DRUG ” is a FP7 - RESEARH POTENTIAL project that aims to the upgrade the Structural Biology capacities of UPAT and to the Establishment of a Center of Excellence for Structure-Based Drug Target chracterization efforts in South-Eastern EU region.
Read the Full Story

“SEE-STRUCT” is structure-oriented module of SEE-DRUG project and aims to the structural elucidation of proteins, enzymes and other interesting drug-targets. The tools offered for these efforts are a state-of-the-art high field NMR instrument of 700 MHz equipped with a highly sensitive probe and crystallization robot.
Read the Full Story

“SEE-PROT” & “SEE-PHARM” are the protein production and the preclinical evaluation of molecules, modules of SEE-DRUG project. The tools offered for these efforts are protein production and characterization facilities an upgraded confocal microscope, an intravital microscope and myographs.
Read the Full Story

NMR
700MHz
High sensetivity probe
Studies in solution
Conformation/Dynamics
3D Structure determination, mobility, protein-protein interaction, protein-drug interactions
Confocal
In-cell imaging
Leica Microscope
Tandem Scanner
Additional laser
Sub-cellular ablation in live cells, protein-complexes, protein drug interactions in vivo
Robot
Crystal growth
Automation
Crystalization trials
Fast & efficient
Crystal growth optimization, screen of protein targes in paraller with NRM Spectroscopy
Microscopes
Myographs
intravital Microscope
In vivo
Ex vivo
Drug screening for anti-inflammatory compounds, evaluation of vasoactive compounds

Stuctural Biology
NMR & Xray
2H/13C/15N labeling
Selective labeling
Crystallization
Conformation dynamics, Modeling Structure determination, 3D solutions & crystal models, Bioinformatics
Molecular Biology
Biochemistry
Drug-targets
Biochemical pathways
Protein production
High-yield protein expression, labeling & isolation for structural studies, protein biochemistry
Imaging
In-cell studies
Functional imaging
Light Microscopy
FRET measurements
Studies of biomolecular functions through Advanced Light Microscopy, Sub-cellular ablations
Pharmacology
Inflammation
Vascular Biology
Cell-based assays
Signaling pathways
Monitoring cell processes (migration. proliferation, apoptosis), in vivo & ex vivo assays

Expertise Exchange
Twinnings
Knowledge transfer
Best practices
New applications
Exchange of researchers between UPAT and partnering organizations - EU Centers of excellence
Events
Workshops
Structural Biology
Biochemistry
Imaging
Workshops and scientific meetings will be organized at UPAT, Advanced training course in NMR
Dissemination
Conferences
Biomolecular NMR
Life Sciences
Analysis
Invited talks, Meeting participation & Poster presentations by SEE-DRUG members
Brokerage Events
Info-days
Meet the expers
Links with industry
Regional Authorities
Colloquia with invited speakers from academia or industry and private sector stakeholders

SEE-STRUCT


“SEE-STRUCT” is structure-oriented module of SEE-DRUG project and aims to the structural elucidation of proteins, enzymes and other interesting drug-targets. The tools offered for these efforts are a state-of-the-art high field NMR instrument of 700 MHz equipped with a highly sensitive probe and crystallization robot.

SEE-STRUCT: Conformational dynamics, interactions & biophysical characterization, to provide atomic-level insight on individual proteins and protein-protein/drug candidates interaction, to identify new protein-protein interactions and to investigate the physicochemical properties (dynamics, affinity, kinetics, etc) of these interactions, based on NMR methods, automated crystallization, crystal structure determination and other complementary techniques. Additionally, NMR is a well-established key technology at the forefront of structural biology and is a particularly versatile tool. NMR analysis is equally valuable in elucidating dynamical properties of individual or interacting biomolecules that can be further tested through complementary tools, such as cell-based assays. In addition, NMR can distinguish and characterize primary or secondary interaction sites, determine the binding affinity of substrates, monitor changes in the conformation or the dynamic properties and overall contribute to the structure-based functional characterization of biomolecules. Moreover, NMR is also applied to monitor the thermodynamic along with the kinetic parameters of protein-protein interaction, an understanding that is crucial in going up a level in comprehending complex interactions in a systems biology context. The installation of the 700 MHz NMR instrument, suitable for proteins >20 kDa and equipped with a cryogenically cooled probe through SEE-DRUG will provide unprecedented possibilities to Greek academic/research staff and industrial stakeholders, such as: (a) in-depth, atomic-level insight into the structure, (b) high resolution and sensitivity for analysis of a variety of fluids (food, olive oil, wine, biofluids, etc.), (c) fast acquisition, allowing real-time NMR use to monitor dynamic processes, and (d) high-throughput structure determination. A crystallization robot will be strongly coupled with the NMR facility. Initial screening of protein-targets with NMR (to confirm, folding, oligomerization state, solubility etc.) will be followed by crystallization screening conditions in order to produce protein-substrate/drug complexes. The coupling of the two instruments will be time and cost-efective since it will reduce the faulse attempts to crystallize structureless or self-aggregating proteins.


Upcoming Events

Hot off the Press

porn /div> The NMR Structure of ARKADIA RING Domain (Proteins 2012, 50, 1484-89)

H2S and NO in angiogenesis & vasodilatation (PNAS 2012 in the press)

Site Statistics

mod_vvisit_countermod_vvisit_countermod_vvisit_countermod_vvisit_countermod_vvisit_countermod_vvisit_counter
mod_vvisit_counterToday10
mod_vvisit_counterYesterday32
mod_vvisit_counterThis week357
mod_vvisit_counterLast week338
mod_vvisit_counterThis month1271
mod_vvisit_counterLast month1442
mod_vvisit_counterAll days184273

We have: 8 guests online
Your IP: 54.80.209.254
 , 
Today: Oct 21, 2017
University of Patras General Info, Services, Research Committee, etc.

FP7 Research & Innovation – Participants Portal – Calls for applications

International Meetings & Conferences

Journal Club

  • 01 Jan 2012 Starting data of SEE-DRUG project

  • 23 Feb 2012 Kick-off meeting

  • 23 Apr2012 External Advisory Board meeting

  • 29 Apr 2013 700MHz NMR installed